Question: Do I have the Alu insert PV92 on my 16th chromosome; determined through polymerase chain reaction and gel electrophoresis?
•Hypothesis:. HINT,
‘kinda’
spelled out in the test!
•Test: We will isolate DNA from human cheek
cells. We will then amplify a segment of chromosome 16 using PCR. DNA samples
will be separated by different sized fragments using gel electrophoresis.
Finally, using proper staining methods to make the DNA visible using Ethidium
Bromide; we will be able to determine the genotype for the human Alu
insert PV92.It will be +,+ (yes, Alu); -,- (no alu), or + ,-(one alu).
•You will NOT need to include full materials lists, procedures or diagrams for this lab.
–Each
of these section headings should be included and
followed by “See
Gene Connection hand outs.”
–Data is a
Labeled diagram of your DNA photo results after gel electrophoresis and your
observation journal. *Observation
Journal is Key here!
–Analysis will
include a discussion of Possible types of error and how they could have
affected your results.
–Conclusion as
per rubric in paragraph format.
No comments:
Post a Comment