Thursday, December 20, 2012
Merry CHristmas, Happy Holidays
To All my students and their Families, I wish you a Merry Christmas and a very happy Holiday Season! See you next Year!
Friday, December 14, 2012
Biology AluPV92 pcrDNA lab
•
Alu insert PV92Background:
– This
experiment examines PV92, a human-specific Alu insertion on chromosome
16.
– The
PV92 genetic system has only two alleles indicating the presence (+) or absence
(-) of the Alu on each of the paired chromosomes.
– This
results in three PV92 genotypes (++, +-, or --).
– The
+ and – “alleles” can be separated by size using gel electrophoresis.
•
The
Alu insert is 300 bp in length; + allele
is 715 bp in length; - allele is 415 bp in length
Question: Do I have the Alu insert PV92 on my 16th
chromosome; determined through polymerase chain reaction and gel
electrophoresis?
•
Hypothesis:. HINT, ‘kinda’ spelled out
in the test!
•
Test: We will isolate DNA from human
cheek cells. We will then amplify a segment of chromosome 16 using PCR. DNA
samples will be separated by different sized fragments using gel
electrophoresis. Finally, using proper staining methods to make the DNA visible
using Ethidium Bromide; we will be able to determine the genotype for the human
Alu insert PV92.It will be +,+ (yes, Alu); -,- (no alu), or + ,-(one alu).
•
You will NOT need to include full materials
lists, procedures or diagrams for this lab.
Each of these section headings should be included and
followed by “See Gene Connection hand outs.”
Data is a Labeled diagram of your DNA photo results after
gel electrophoresis and your observation journal. *Observation Journal is Key here!
Analysis will include a discussion of Possible types
of error and how they could have affected your results.
•
Conclusion as per rubric in paragraph
format.
Wednesday, December 12, 2012
Earthscience/biology Current Events extra credit format
You will choose an article or science
related video of over 2 pages or 45 minutes on science current event. It will
fall into one (or more) of these categories:
·
Scientific
discovery – for example, scientists find comets may
have delivered the water found in earth’s oceans.
·
Science-related
event – for example, the Japan
earthquake/tsunami/nuclear plant meltdown; the launch of a space probe.
·
A cultural
change/happening that involves science/technology – for
example, bicyclists’ clothing that can act as a radar gun to show drivers how
fast they’re moving.
·
Science in
public policy – for example, a bill or law that deals
with science (cap on carbon emissions).
The article must be timely, meaning it’s
been published within the past one/two months. Finally, technology articles are
okay, but I will not accept any product announcements or reviews.
Create a write-up of your current event,
summarizing the story and answering key questions. You will include a copy of
the story you are citing or the golden page format for the video, movie or
program.
Science current events will:
Cite the
news source, using the Oceana Golden page format for citations found on
the Oceana website. Magazine Article:
Perlman, Jeff. “Sultan of Swat.” Sports Illustrated. 12 February 2001: 54-59.
Newspaper Article:
* Taylor, Michael. “Potholes Scar Roads Across Region.” San Francisco Chronicle. 23 October 2005:
* Give the title of the article, author’s
name, and article date within the body of the summary.
* Summarize the event in your own words,
using complete sentences. Spelling and grammar count.
* Your summary will answer the following
questions:
·
Who/what is the
subject of the story?
·
Where did it happen?
·
When did it happen?
·
How did it happen?
·
Why did the event
happen, or why was the event important enough to make the news?
·
Why did you choose
this article?
·
What did you learn
from the article, and what are your thoughts about this event/issue?
*CONNECTION AND COMMENT RELATED TO ONE ESSENTIAL
QUESTION:
·
How does this event
affect the world or region where it occurred?
·
How does this
discovery advance science?
·
What does the
discovery/event mean for our society?
·
What does this
discovery/event mean for future work/the environment/humanity/etc.?
·
If the story is about
a law or policy, how does the policy or law affect science/society?
Below are several acceptable web sites and
sources for science news stories. If you wish to use a source not listed below,
please check with me first.
Popular Science
New Scientist (News)
Wired Science
New York Times (Science Times)
Discover Magazine
National Geographic (News)
BBC Science/Environment
http://www.bbc.co.uk/news/science_and_environment/
NPR - Science News
Boing Boing (Science)
Grist
Good (Environment, Food, Health, Technology,
Transportation)
The Guardian (UK) Science
Scientific American (Often
written for college level or higher)
Reuters Science
Biology : ALuPV92 PCRDNA Lab
PCR/DNA Lab: Alu
PV92
Question: Do I have the Alu insert PV92 on my 16th
chromosome; determined through polymerase chain reaction and gel
electrophoresis?
Hypothesis:. HINT, ‘kinda’ spelled out in the
test!
Test: We will isolate DNA from human cheek cells. We
will then amplify a segment of chromosome 16 using PCR. DNA samples will be
separated by different sized fragments using gel electrophoresis. Finally,
using proper staining methods to make the DNA visible using Ethidium Bromide;
we will be able to determine the genotype for the human Alu insert PV92.It will
be +,+ (yes, Alu); -,- (no alu), or + ,-(one alu).
*You will NOT need to include full materials lists, procedures
or diagrams for this lab.
Each of these section headings, materials and procedures, should be included and followed by “See
Gene Connection hand outs.”
Data is a Labeled diagram of your DNA photo results
after gel electrophoresis and your observation journal. *Observation Journal is Key here!
Analysis will include a discussion of Possible types
of error and how they could have affected your results.
Conclusion as per lab rubric in paragraph format.
Friday, November 9, 2012
Earth science Mass of a rubber stopper
Do not
write on this handout, make an RD in your notebook, the FD will be on a
separate sheet(s) of paper one for each student.
Title:
Just how much Rubber is in a Rubber Stopper?
Question:
How will the rubber stopper’s actual metric mass compare (more, less or same mass) to the converted
mass taken from posted English standard measurements?
Hypothesis:
The Rubber stopper’s actual mass will be________________ then/as the
posted mass.
Test:
By using a
metric balance to determine the actual mass of a Rubber stopper, we can convert
ounces to grams for the posted mass and compare to the actual mass found using
a scale.
Materials:
metric balance (triple beam pan balance), rubber stoppers of varying
size, (calculator)
Procedures:
- Convert the posted mass from oz → g
using the following formula 1oz. = 28.3 g
- Take the mass measurement of the rubber
stopper on the metric balance.
- Do this for four different rubber
stoppers with different posted masses (each table member should do all
parts of the experiment for at least one sinker)
- Subtract the measured mass in grams
from the posted mass in grams to determine the relative error. You may disregard any negative numbers9
take an absolute value of your result).
- Take the relative error in grams
and divide by the converted posted mass, in grams, and multiply by 100 to
determine the percent error.
Data:
Rubber
stopper #
|
Posted mass
Converted (g)
|
Measured mass
(g)
|
Relative
Error
|
% error
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Observations:
Analysis:
include your calculations and math work for doing the oz-g conversions,
relative and percent error.
Make a Bar
graph of actual versus converted mass values of the rubber stoppers used.
Conclusion: (restate
the hypothesis, explain how you answered the question or not and compare
numeric results to prove or refute your hypothesis, discuss observations as
possible errors and propose a next step and who outside of this science class
might care about your results)
Thursday, November 1, 2012
ES volumetrics 3* data
object | Accepted values | 3* | 400ml pb/250ml gb/100ml gc | ||||||||||||
1* | 157 cm3 | grp 1 | grp 2 | grp 3 | grp 4 | grp 5 | grp 6 | grp 7 | grp 8 | grp 9 | |||||
2* | 159 cm3 | ML | ML | ML | ML | ML | ML | ML | ML | ML | |||||
3* | 140 cm3 | #1 | 160/175/155 | 160/175/155 | 180/150/157 | 180/150/152 | 160/150/153 | ||||||||
4* | 165 cm3 | #2 | 180/175/155 | 160/150/158 | 160/150/159 | 180/150/158 | 160/150/156/ | ||||||||
5* | 251 cm3 | #3 | 120/150/140 | 160/150/141 | |||||||||||
6* | 237 cm3 | #4 | 140/125/100 | 160/150/137 | 140/125/133/ | ||||||||||
7* | 160 cm3 | #5 | 260/250/252 | 280/275/246 | 280/250/247 | 260/250/253/ | |||||||||
8* | 257 cm3 | #6 | 260/250/235 | 240/250/236 | 240/225/234 | 240/200/200 | 240/250/228 | 240/250/221/ | |||||||
9* | 249 cm3 | #7 | 160/175/158 | 180/150/161 | 160/150/160 | 160/150/130 | 180/150/162 | 160/175/156 | 160/150/153/ | ||||||
#8 | 280/275/255 | 260/250/2583 | 260/250/258 | 280/250/284 | 260/250/252/ | ||||||||||
#9 | 250/225/245 | 260/250/242 | 260/250/264 | 240/250/244 | 240/250/220 | 260/250/248/ | |||||||||
relative error = accepted value - measured average |
ES volumetrics class data 5*
object | Accepted values | 5* | 400ml pb/250ml gb/100ml gc | |||||||||||
1* | 157 cm3 | grp 1 | grp 2 | grp 3 | grp 4 | grp 5 | grp 6 | grp 7 | grp 8 | grp 9 | ||||
2* | 159 cm3 | object | ML | ML | ML | ML | ML | ML | ML | ML | ML | |||
3* | 140 cm3 | #1 | 160/175/155 | 160/175/155 | 180/150/157 | 180/150/152 | 160/150/153 | |||||||
4* | 165 cm3 | #2 | 180/175/155 | 160/150/158 | 160/150/159 | 180/150/158 | 160/150/156/ | |||||||
5* | 251 cm3 | #3 | 120/150/140 | 160/150/141 | ||||||||||
6* | 237 cm3 | #4 | 140/125/100 | 160/150/137 | 140/125/133/ | |||||||||
7* | 160 cm3 | #5 | 260/250/252 | 280/275/246 | 280/250/247 | 260/250/253/ | ||||||||
8* | 257 cm3 | #6 | 260/250/235 | 240/250/236 | 240/225/234 | 240/200/200 | 240/250/228 | 240/250/221/ | ||||||
9* | 249 cm3 | #7 | 160/175/158 | 180/150/161 | 160/150/160 | 160/150/130 | 180/150/162 | 160/175/156 | 160/150/153/ | |||||
#8 | 280/275/255 | 260/250/2583 | 260/250/258 | 280/250/284 | 260/250/252/ | |||||||||
#9 | 250/225/245 | 260/250/242 | 260/250/264 | 240/250/244 | 240/250/220 | 260/250/248/ | ||||||||
relative error = accepted value - measured average | ||||||||||||||
for 400 ml plastic beaker | ||||||||||||||
Ex #5: 251 - 260 = -9 |
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