PCR/DNA Lab: Alu
PV92
Question: Do I have the Alu insert PV92 on my 16th
chromosome; determined through polymerase chain reaction and gel
electrophoresis?
Hypothesis:. HINT, ‘kinda’ spelled out in the
test!
Test: We will isolate DNA from human cheek cells. We
will then amplify a segment of chromosome 16 using PCR. DNA samples will be
separated by different sized fragments using gel electrophoresis. Finally,
using proper staining methods to make the DNA visible using Ethidium Bromide;
we will be able to determine the genotype for the human Alu insert PV92.It will
be +,+ (yes, Alu); -,- (no alu), or + ,-(one alu).
*You will NOT need to include full materials lists, procedures
or diagrams for this lab.
Each of these section headings, materials and procedures, should be included and followed by “See
Gene Connection hand outs.”
Data is a Labeled diagram of your DNA photo results
after gel electrophoresis and your observation journal. *Observation Journal is Key here!
Analysis will include a discussion of Possible types
of error and how they could have affected your results.
Conclusion as per lab rubric in paragraph format.
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